Natural Bio-Compounds from Ganoderma lucidum and Their Beneficial Biological Actions for Anticancer Application: A Review

Ganoderma lucidum (G. lucidum) has been known for many centuries in Asian countries under different names, varying depending on the country. The objective of this review is to investigate the scientific research on the natural active bio-compounds in extracts obtained from G. lucidum with significant biological actions in the treatment of cancer. This review presents the classes of bio-compounds existing in G. lucidum that have been reported over time in the main databases and have shown important biological actions in the treatment of cancer. The results highlight the fact that G. lucidum possesses important bioactive compounds such as polysaccharides, triterpenoids, sterols, proteins, nucleotides, fatty acids, vitamins, and minerals, which have been demonstrated to exhibit multiple anticancer effects, namely immunomodulatory, anti-proliferative, cytotoxic, and antioxidant action. The potential health benefits of G. lucidum are systematized based on biological actions. The findings present evidence regarding the lack of certainty about the effects of G. lucidum bio-compounds in treating different forms of cancer, which may be due to the use of different types of Ganoderma formulations, differences in the study populations, or due to drug–disease interactions. In the future, larger clinical trials are needed to clarify the potential benefits of pharmaceutical preparations of G. lucidum, standardized by the known active components in the prevention and treatment of cancer.


Introduction
Ganoderma lucidum (G.lucidum), (Fr.)Karst is a medicinal mushroom known in traditional Asian medicine under different names depending on the country: Lingzhi (China), Reishi, and Mannentake (Japan), Linh chi (Vietnam), and Yeong Ji or Yung Gee (Republic of Korea) [1].It is considered a source of longevity and health promotion [2][3][4].The traditional medicinal uses of these mushrooms in Chinese and Japanese folk medicine as health remedies and herbal supplements are widely recognized [5][6][7].The knowledge and use of medicinal mushrooms have preoccupied researchers, who have developed valuable studies on their bioactive components and their importance [8][9][10][11][12].According to the World Health Organization, it has been estimated that around three-quarters of the world's population relies on traditional medicines to maintain their health [13].In traditional oriental medicine, G. lucidum has been used to treat several diseases, such as asthma, bronchitis, arthritis, hypertension, insomnia, diabetes, liver disease, nephritis, and cancer [14][15][16].In ancient Chinese medicine, G. lucidum has been used for longevity and as an anticancer and antioxidant agent [17][18][19][20].In the last 30 years, data have been reported on the chemical composition of G. lucidum extracts, justifying their biological activity and numerous health benefits.Among the most important bioactive compounds are polysaccharides and triterpenes [21][22][23][24][25][26].Numerous other bioactive metabolites, such as proteins, peptides, sterols, lectins, adenosine, vitamins, and metals, have also been identified [27][28][29][30][31].However, it should be noted that there are differences in the composition of G. lucidum products due to cultivation conditions, the extraction process of the bio-compounds, and the origin or part of the fungus used (fruiting body, mycelium, or spores) [14,28,30].Nevertheless, many bioactive compounds in G. lucidum exhibit antioxidant, antitumor, and anti-inflammatory properties [32][33][34][35][36][37].Such benefits have been investigated in multiple studies on their effect against prostate cancer, ovarian cancer, hepatocellular carcinoma, the induction of apoptosis in colon cancer, and the inhibition of angiogenesis [38][39][40][41][42][43].Different immunomodulatory activities have been reported [44][45][46][47].Other researchers have also reported studies on antioxidant and antioxidative stress actions [48][49][50].With the development of modern research techniques to identify the chemical compositions of G. lucidum compounds, data may be accessible for use in medical research.A detailed understanding of these biological mechanisms could greatly influence and extend their benefits to human health.
The present study aims to review the data on active bio-compounds with important biological actions against cancer.Factors influencing the composition of the G. lucidum fungus, such as growing conditions (in the wild, in deciduous forests, or in special crops) and territorial areas, are analyzed.The characteristics of the G. lucidum fungus, as well as the chemo-bioactive compounds identified in G. lucidum with anticancer activities, its biological activities against cancer, and its toxicity and safety, are described in separate sections.The mechanisms of anticancer effects, including other biological activities that contribute to the fight against cancer, such as immunomodulatory, antioxidant, and cytotoxic actions, are also presented.Some negative cases in which G. lucidum bio-compounds were administered to patients undergoing treatment for several conditions are included as well.In these patients, the effects of G. lucidum treatments were not beneficial due to the occurrence of adverse effects.

Characteristics of Ganoderma lucidum Fungus 2.1. Description and Spread of the Fungus
The first description of Ganoderma lucidum (Curtis) P. Karst.was made by Curtis in England, and this description was officially recorded by Fries [51].Initially, Cao et al. claimed that this fungus was already known as "Lingzhi", a medicinal fungus identified and used in China for more than 2000 years [52].Later, based on molecular studies, it was established that the East Asian medicinal mushroom is a different species from the G. lucidum mushroom [52].After morphological and molecular examinations, Wang et al. confirmed that the Lingzhi species from China are related to G. lucidum from the UK and to other Ganoderma species [53].Kwon et al. conducted phylogenetic analyses of Ganoderma species and showed that there are 62 strains of Ganoderma [54].According to the taxonomic classification established by Nahata A., the species Ganoderma lucidum (Curt: Fr.) Karst belongs to the kingdom Fungi, phylum Basidiomycota, class Agaricomycetes, order Polyporales, family Ganodermataceae, genus Ganoderma, and species lucidum [55].G. lucidum from the UK and other related Ganoderma species have also been morphologically and molecularly examined by different researchers who have conducted important studies, such as Kim et al., Park et al.,.Several phylogenetically related Ganoderma species have been found in North America, Europe, and Asian countries [56,58].Gottlieb et al. also performed molecular and morphological studies on the Ganoderma species collected from South America, followed by Eyssartier et al. from France [59,60].In 2017, Copot et al. identified the fungus G. lucidum in hilly and mountainous areas in Romania, specifically in oak forests [61].The description of G. lucidum collected from Romania highlighted the mushroom cap, which is kidney-shaped and can be up to 20 cm in diameter, ranging in color from red to pinkish-brown when mature [61].Towards the edges, the coloration of the cap ranged from bright yellow to white.The spore print is brown (see Figure 1a,b) [61].Figure 1c shows the fungus G. lucidum (Reishi or Lingzhi) adapted from studies by Parepalli et al. [62].
Antioxidants 2023, 12, x FOR PEER REVIEW 3 of 36 Polyporales, family Ganodermataceae, genus Ganoderma, and species lucidum [55].G. lucidum from the UK and other related Ganoderma species have also been morphologically and molecularly examined by different researchers who have conducted important studies, such as Kim et al., Park et al., and Liao et al. [56][57][58].Several phylogenetically related Ganoderma species have been found in North America, Europe, and Asian countries [56,58].Gottlieb et al. also performed molecular and morphological studies on the Ganoderma species collected from South America, followed by Eyssartier et al. from France [59,60].In 2017, Copot et al. identified the fungus G. lucidum in hilly and mountainous areas in Romania, specifically in oak forests [61].The description of G. lucidum collected from Romania highlighted the mushroom cap, which is kidney-shaped and can be up to 20 cm in diameter, ranging in color from red to pinkish-brown when mature [61].Towards the edges, the coloration of the cap ranged from bright yellow to white.The spore print is brown (see Figure 1a,b) [61].Figure 1c shows the fungus G. lucidum (Reishi or Lingzhi) adapted from studies by Parepalli et al. [62].

Data on G. lucidum Cultivation
G. lucidum can also grow in greenhouses under controlled conditions.Data on cultivation methods have been reported in the literature, such as the study of Boh et al., where different biotechnological cultivation methods are presented [14].As G. lucidum is rare in nature, cultivation in greenhouses is practiced using two methods: cultivation of fruiting bodies on wooden logs or on sawdust bags (or large plastic bottles) [14].The main cultivation methods to produce G. lucidum (fruiting body and mycelia) are presented in Figure 2 [14].

Data on G. lucidum Cultivation
G. lucidum can also grow in greenhouses under controlled conditions.Data on cultivation methods have been reported in the literature, such as the study of Boh et al., where different biotechnological cultivation methods are presented [14].As G. lucidum is rare in nature, cultivation in greenhouses is practiced using two methods: cultivation of fruiting bodies on wooden logs or on sawdust bags (or large plastic bottles) [14].The main cultivation methods to produce G. lucidum (fruiting body and mycelia) are presented in Figure 2 [14].
Polyporales, family Ganodermataceae, genus Ganoderma, and species lucidum [55].G. lucidum from the UK and other related Ganoderma species have also been morphologically and molecularly examined by different researchers who have conducted important studies such as Kim et al., Park et al.,.Several phylogenetically related Ganoderma species have been found in North America, Europe, and Asian countries [56,58].Gottlieb et al. also performed molecular and morphological studies on the Ganoderma species collected from South America, followed by Eyssartier et al. from France [59,60].In 2017, Copot et al. identified the fungus G. lucidum in hilly and mountainous areas in Romania, specifically in oak forests [61].The description of G. lucidum collected from Romania highlighted the mushroom cap, which is kidney-shaped and can be up to 20 cm in diameter, ranging in color from red to pinkish-brown when mature [61].Towards the edges, the coloration of the cap ranged from bright yellow to white.The spore print is brown (see Figure 1a,b) [61].Figure 1c shows the fungus G. lucidum (Reishi or Lingzhi) adapted from studies by Parepalli et al. [62].

Data on G. lucidum Cultivation
G. lucidum can also grow in greenhouses under controlled conditions.Data on cultivation methods have been reported in the literature, such as the study of Boh et al., where different biotechnological cultivation methods are presented [14].As G. lucidum is rare in nature, cultivation in greenhouses is practiced using two methods: cultivation of fruiting bodies on wooden logs or on sawdust bags (or large plastic bottles) [14].The main cultivation methods to produce G. lucidum (fruiting body and mycelia) are presented in Figure 2 [14].Various other cultivation methods have also been practiced.For example, Nithya et al. conducted research on the selection of an ideal material to grow G. lucidum fungus by testing wood retting, wheat bran, sorghum, and sorghum grains combined with chalk dust and gypsum [63].Adongbede et al. (2021) used indigenous hardwoods supplemented with rice and wheat bran as substrates for growing G. lucidum in Nigeria, where the fungus is not naturally available [64].Matute et al. (2002) grew G. lucidum in bags using sunflower hulls as a nutrient source [65].Yang et al. (2003) used carbohydrate and nitrogen-rich residues from a rice bran distillery to grow G. lucidum in polypropylene bags [66].Hsieh et al. (2004) used soybean residues as a nutrient for growing G. lucidum in polypropylene bags [67].Chang et al. (2006) reported studies on the optimization of growth methods for G. lucidum [68].It is worth noting that cultivation conditions and substrate composition influence the biochemical composition of G. lucidum fungi qualitatively and quantitatively, as confirmed by Baskar et al. in 2011 through their studies [69].

Chemical Bioactive Compounds Identified in G lucidum with Anticancer Actions
In the last 30 years, numerous studies have been conducted highlighting the nutritional potential of G. lucidum mushrooms [30].Research on the biochemical composition of this mushroom has led to the identification of several categories of compounds with healthpromoting biological activity, as reported by Ahmad et al. [7,13].

Proximate Composition for G. lucidum
The values of the bioactive compound classes differ quantitatively and qualitatively within certain limits, depending on the country and area of origin of the fungus and whether it is cultivated or naturally occurring in the forest.In the case of cultivated mushrooms, bioactive compounds depend on the nutrient richness of the substrate and environmental factors, such as humidity and temperature [14].Most phytochemical reports show that, with regard to the chemical composition, the fruiting body of G. lucidum consists of 90% water and 10% different compounds, as presented in Table 1, according to Mau et al. [70].Table 1 summarizes the information on the biochemical composition of the fungus, as noted by multiple researchers.The content analysis of the bioactive compound classes includes data on moisture, ash, water-soluble protein content, total lipid content, total carbohydrate content, and dietary fiber content.From Bangladesh, data were reported by Rahman et al. (2020), El Sheikha (2022), and Roy et al. (2018) [21,30,71].The interest in the nutritional potential of G. lucidum in Taiwan was proven by studies conducted by Mau et al. (2001) [70].The total fatty acid content was reported only by Fraile-Fabero et al. for G. lingzhi from China and G. lucidum from mushroom crops from Madrid, Spain, in 2021 [72].Comparable data were also reported by Parapelli et al. (2021) for G. lucidum from India and by Ogbe et al. (2013) from Nigeria [73,74].Additional studies were published by Wachtel-Galor et al. (2011), by Paterson et al. (2006), and by Garuba et al. (2000) [75][76][77].The pharmacognostic review of the active compounds isolated from basidiocarp and mycelium of G. lucidum revealed that it contains polysaccharides, triterpenes, vitamins, minerals, sterols, proteins, proteo-polysaccharides, lectins, nucleotides, and fatty acids, as reported by Ahmad (2018) [13].Figure 3 systematizes the categories of biochemical compounds that support the biological activities occurring in the treatment of various cancer tumors [13].

Extraction and Purification of Polysaccharides
The most widely used method for polysaccharide extraction from spores, fruiting bodies, and mycelium is hot-water extraction, as documented by Nie et al. [84].Figure 4 illustrates the extraction of five polysaccharide fractions from G. lucidum [85].The fruiting body of G. lucidum was initially peeled, shredded, and sieved in order to obtain a fine powder.The extraction of Gl-Ps from the mushroom powder was performed with distilled water at 80 °C [85].There are researchers who have used diluted saline solution or alkaline acid solutions for polysaccharide extraction, as reported by Wang et al. (2011).

Extraction and Purification of Polysaccharides
The most widely used method for polysaccharide extraction from spores, fruiting bodies, and mycelium is hot-water extraction, as documented by Nie et al. [84].Figure 4 illustrates the extraction of five polysaccharide fractions from G. lucidum [85].The fruiting body of G. lucidum was initially peeled, shredded, and sieved in order to obtain a fine powder.The extraction of Gl-Ps from the mushroom powder was performed with distilled water at 80  [91][92][93].Ion-exchange chromatography (DEAE-Sepharose Fast Flow), gel filtration, and affinity chromatography were the most useful methods, as highlighted by Choong et al. [85].Figure 4 illustrates the extraction and fractionation steps of polysaccharides from G. lucidum.Five polysaccharide fractions were obtained [85].
Other extraction methods that were applied included microwaves, ultrasound, or enzymatic methods, as in the studies of Lin et 2012) [91][92][93].Ion-exchange chromatography (DEAE-Sepharose Fast Flow), gel filtration, and affinity chromatography were the most useful methods, as highlighted by Choong et al. [85].Figure 4 illustrates the extraction and fractionation steps of polysaccharides from G. lucidum.Five polysaccharide fractions were obtained [85].

Structure of Polysaccharides from G. lucidum
The investigation of the structures and chemical properties of polysaccharides from G. lucidum involves knowledge of the composition of existing monosaccharides, branching structures, types of glycosidic linkages, chain conformations, and molecular weights [18].As investigation techniques have developed, it was found that G. lucidum can have polysaccharides in its composition, either as pure or linked with other proteins or peptides [94][95][96][97][98]. Bhat et al. (2021) showed that homo-glucans from G. lucidum are linear or branched biopolymers, possessing a backbone formed by α-or β-linked (1→3), (1→6)-βglucan and (1→3)-α-glucan glucose units, and may possess side chains attached in different positions [18].Further investigations by different authors explore bioactivities as well.Bao et al. (2002) determined that the structures of the backbone chains are linear or branched bipolymers [94].These possess a backbone consisting of α-or β-linked glucose units [94].In 2006, new structures with heteroglucans were outlined by Cao et al., and new structures with heteropolysaccharides were documented by Sullivan et al. [95,96].

Structure of Polysaccharides from G. lucidum
The investigation of the structures and chemical properties of polysaccharides from G. lucidum involves knowledge of the composition of existing monosaccharides, branching structures, types of glycosidic linkages, chain conformations, and molecular weights [18].As investigation techniques have developed, it was found that G. lucidum can have polysaccharides in its composition, either as pure or linked with other proteins or peptides [94][95][96][97][98]. Bhat et al. (2021) showed that homo-glucans from G. lucidum are linear or branched biopolymers, possessing a backbone formed by αor β-linked (1→3), (1→6)-β-glucan and (1→3)-α-glucan glucose units, and may possess side chains attached in different positions [18].Further investigations by different authors explore bioactivities as well.Bao et al. (2002) determined that the structures of the backbone chains are linear or branched bipolymers [94].These possess a backbone consisting of αor β-linked glucose units [94] 2 illustrates various techniques and methods utilized in the extraction, fractionation, and purification of polysaccharides and provides information about their structure (the backbone and monosaccharide compositions).
Antioxidants 2023, 12, x FOR PEER REVIEW 9 Side-chain branching occurs at C-6 of the main-chain glucosyl residues, as show

Triterpene and Triterpenoid Content of G. lucidum
Triterpenes belong to the class of terpenes that have a molecule consisting of six prene units and are widespread in the plant kingdom [35].Triterpenoids are part of triterpene class, having heteroatoms (usually oxygen atoms) in the molecule.They important bioactive compounds in the composition of G. lucidum, as shown by Wu e (2023) [118].In 2018, Gu et al. revealed that the present triterpenes synthesize struct derived from lanosterol, which has a skeleton with a tetracyclic structure and the mo ular formula C30H48 [119].Terpenoid compounds from G. lucidum that have a C30 b bone and molecular masses between 400 and 600 kDa were studied by Baby et al.Galappaththi et al., who generically named them Ganoderma triterpenoids (G [120,121].Further research on the structure and characterization of triterpenoids from

Triterpene and Triterpenoid Content of G. lucidum
Triterpenes belong to the class of terpenes that have a molecule consisting of six isoprene units and are widespread in the plant kingdom [35].Triterpenoids are part of the triterpene class, having heteroatoms (usually oxygen atoms) in the molecule.They are important bioactive compounds in the composition of G. lucidum, as shown by Wu et al. (2023) [118].In 2018, Gu et al. revealed that the present triterpenes synthesize structures derived from lanosterol, which has a skeleton with a tetracyclic structure and the molecular formula C 30 H 48 [119].Terpenoid compounds from G. lucidum that have a C30 backbone and molecular masses between 400 and 600 kDa were studied by Baby et al. and Galappaththi et al., who generically named them Ganoderma triterpenoids (Gl-Ts) [120,121].Further research on the structure and characterization of triterpenoids from G. lucidum that elucidates their biological activities was published in 2022 by Cör et al. and Lin et al. [122,123].2023) extensively reported on the diverse types of Gl-Ts identified from the mycelia, fruit body, and spores of G. lucidum, showcasing distinct structural variations, including alcohols, aldehydes, ketones, acids, esters, and various other substituents positioned differently [118].Xia et al. (2014) conducted an in-depth analysis of the skeletal structure of G. lucidum, revealing that a majority of these terpenoids consist of 30 carbon atoms [135].Using information derived from Wu et al., in Figure 6, the structures of a typical terpenoid skeleton are depicted, illustrating the numbered positions corresponding to the matched carbon atoms alongside 10 additional terpenoid structures exhibiting diverse substituents [118].
In Figure 6, illustrating the typical terpenoid skeleton structure, distinct substituents are evident at positions C-3, C-7, C-11, C-12, C-15, C-20, and C-27.Fatmawati et al. (2010) elucidated the structure of a new terpenoid, Ganoderic acid Df, featuring a β-hydroxy substituent at the C-11 position, distinguishing it from all other compounds characterized by a carbonyl group at the same position [136].At the C-3 position, potential substituents include the β-hydroxy, carbonyl, and β-acetoxy groups.At C-20, a single carbon atom can host two substituents, which may be the methyl or hydroxyl groups or even hydrogen.Wu et al. demonstrated that at the C-25 position, various carboxyl groups, such as formyl, acetyl, or butyryl, can be found [118].Additionally, Sharma et al. (2019) presented data on triterpenes from G. lucidum, characterized by isoprene units in their composition, featuring a C30 skeleton structure of ganoderic acids, aldehydes, esters, alcohols, lactones, glycosides, ketones, and molecular masses ranging from 400 to 600 g/mol [137].Koo et al. (2019) identified a new compound with a lanostane triterpenoid structure named Ganosidone A, which, along with eight other derivatives, was investigated for its cancer chemopreventive potential [138].Concerning the molecular configuration of ganoderic acids, Cör et al. (2022) documented the structures illustrated in Figure 7 [122].
tioned differently [118].Xia et al. (2014) conducted an in-depth analysis of the skeletal structure of G. lucidum, revealing that a majority of these terpenoids consist of 30 carbon atoms [135].Using information derived from Wu et al., in Figure 6, the structures of a typical terpenoid skeleton are depicted, illustrating the numbered positions corresponding to the matched carbon atoms alongside 10 additional terpenoid structures exhibiting diverse substituents [118].3 displays the values for mushroom compositions reported by various authors, expressed in milligrams per 100 g (mg/100 g), parts per million (ppm), and percentages (%) [30,71,74,153,156].3 displays the values for mushroom compositions reported by various authors, expressed in milligrams per 100 g (mg/100 g), parts per million (ppm), and percentages (%) [30,71,74,153,156].
Similar data were also presented by  [159].In 2008, Falandysz J. reported the existence of selenium in the composition of some antioxidant enzymes with protective actions that are involved in antitumor effects [160].In their study, conducted in 2000, Chiu et al. identified the presence of germanium in the Ganoderma extract.Their research encompassed an assessment of genotoxicity and antigenotoxicity related to this finding [161].Du et al. (2008) studied the positive effect of selenium on the immune regulation activity of G. lucidum, and the involvement of this element in anticancer activities was documented [162].[163].The role of sterols is important in the body's metabolism in regulating some hormonal and immune system functions.Akihisa et al. (2007) studied the effects of sterols and triterpene acids from G. lucidum on anti-inflammatory and antitumor actions [164].In 2011, the sterol content of G. lucidum was researched by Liu et al., who analyzed it qualitatively and quantitatively using HPLC methods [165].In 2015, Baby et al. showed that sterols in G. lucidum can be classified based on their skeletons [120].[166][167][168][169].In 2015, Saard et al. studied crude proteins from G. lucidum (both mycelia and fruiting bodies) for their antioxidant actions [170].In 2021, Fraile-Fabero et al. conducted studies on proteins from G. lucidum [72].Additional research by Sun et al. in 2004 revealed the existence of compounds with a polysaccharide-peptide complex structure along with phenolic components with antioxidant potential in G. lucidum [171].Ji et al. (2007) further documented the existence of proteo-polysaccharides in Ganoderma lucidum.They studied the immunomodulatory effects, emphasizing the anticancer properties of this fungus [172].The immunomodulatory properties of LZ-8 protein and polysaccharides were studied by Yeh et al. in 2010 and Girjal et al. in 2012 [173,174].Zhong et al. (2015) conducted research on the involvement of the peptide-polysaccharide complex from G. lucidum in oxidative stress [175].Subsequent data on the presence of proteins in G. lucidum and their biological actions were published by Kumakura et    The antioxidant properties of G. lucidum were analyzed using several methods, including the reporting of DPPH and ABTS radical scavenging activities, as well as the FRAP assay.The results were expressed in units of measurement.These units vary according to the different experimental methods used.However, the importance of the results lies in the unequivocal demonstration of the antioxidant activity possessed by the constituents found in G. lucidum (see Table 7).2019) demonstrated a strong correlation between the antioxidant capacities measured using DPPH, ABTS, and FRAP assays and the content of polyphenolics and triterpenoids in G. lucidum [190].Similar studies have also been published by Saltarelli et al. in 2015 [193].The properties of compounds in G. lucidum and their effects on antioxidant activity were also documented by Tang et [194][195][196][197]. Nucleotides are monomeric units that consist of a base (purine or pyrimidine) and one or more phosphate groups.Nucleotides are formed from nucleosides by phosphorylation under the action of kinases.Nucleosides are glycosyl amines derived from a nitrogenous base and a ribose or deoxyribose.These compounds have been shown to contain uridine and uracil, both of which are capable of reducing elevated serum aldolase levels.The watersoluble fraction of Ganoderma suppresses platelet aggregation.Cheung et al. have carried out studies on the identification and role of bases and nucleoside markers [198].Gao et al. (2007) identified six nucleobases qualitatively in fruiting body samples of G. lucidum and G. sinense [199].Additional studies of the distribution of nucleotides and nucleobases were also carried out by Yuan et al. in 2008 andChen et al. in 2012, who were able to document 16 nucleotides and nucleobases in the Ganoderma species [200,201].Phan et al. (2018) demonstrated in their work that nucleobases, nucleosides, and nucleotides found in fungi play crucial roles in regulating various physiological processes in the human body through purinergic and/or pyrimidine receptors.[202].In 2022, Sheng et al. analyzed 10 target compounds from 23 batches of Ganoderma samples from different regions of China and demonstrated that the geographical origin of the fungi might be the exclusive factor affecting the accumulation of nucleosides and nucleobases in the Ganoderma spp.mycelium [203].

Fatty Acid Content
The total lipid content of G. lucidum has been reported in several studies by Rahman et al. (2020), El Sheikha (2022), Roy (2018), and Ogbe et al. (2013) [21,30,71,74].Comparative data for the fatty acid content of carpophores from G. lingzhi (from industrial cultures in China) and G. lucidum (from cultures in Madrid, Spain) were described by Fraile-Fabero et al. in 2021 [72].Fraile-Fabero et al. found that G. lingzhi contains the highest percentage of arachidic acid, followed by the margaric, behenic, margaroleic, lignoceric, and cis-vaccenic acids, while G. lucidum contains the highest percentage of α-linolenic acid, followed by the myristic, stearic, capric, erucic, nervonic, elaidic, octadecatrienoic, octadecadienoic, and eicosatrienoic acids [72].Data on the presence of essential fatty acids in G. lucidum were also presented by Hossain et al. in 2007 [204].Stojković et al. (2014) conducted comparative studies between the G. lucidum species from Serbia and China [205].Lin et al. (2017) reported hydroxy fatty acids (HFA) present in the molecular species of acylglycerols of G. lucidum [206].Salvatore et al. (2020) identified fatty acid methyl esters (FAMEs) in the triglycerides of G. lucidum spores [207].Table 8 displays the composition similarities between the two species.[72].Phytochemical reports over the past four decades indicate the presence of 279 bioactive secondary metabolites (Wasser et al.), along with over 200 polysaccharides and polysaccharide-protein complexes (Baby et al.), and more than 30 steroidal compounds (Wasser et al.) within the chemical composition of G. lucidum.These compounds play a pivotal role in the development of various biological actions.[120,208,209].

Biological Activities against Cancer
The biocomponents present in G. lucidum contribute to the numerous biological actions of this medicinal mushroom, making it applicable in medical contexts for combating cancer.Both polysaccharide compounds (Gl-Ps) and triterpene compounds (Gl-Ts) have been shown to exhibit anticancer activities.Over a 25-year period, numerous studies have investigated the anticancer effects of G. lucidum compounds, including those reported by Wang et [13,153,192,210].Our aim is to review the most important studies and systematize these data on the possible mechanisms.

Anticancer Action
The anticancer activity attributed to Gl-Ps was studied by Cao et al. in 2002 and2003 [211,212].Many other studies have demonstrated the effect of Gl-Ps on cytokines, including those by Chen et al. and Zhu et al. [213,214].In 2007, Zhu et al. demonstrated the beneficial immunological effects in mice attributed to Gl-Ps extracts administered at low doses [215].You et al. studied the effects of Gl-Ps on antioxidant enzymes in ovarian cancer in rats, while Xu et al. conducted research on mediating the immunomodulatory, cytotoxic, and anti-angiogenic effects of Gl-Ps [216,217].Polysaccharides have been identified as a distinct class of compounds present in G. lucidum compositions, demonstrating anticancer activity by stimulating host immune function, as reported by Ahmad F., Sun et al., and Wiater et al. [213,218,219].In 2012, Zhang et al. reported the effects of Gl-Ps on suppressing hepatocyte proliferation in rats [220].Pan et al. reported the effects of Gl-Ps on rats with gastric cancer, inducing enhanced immunity and antioxidant activity [103].The study by Suarez-Arroyo et al. in 2013 explored inflammatory breast cancer (IBC) and the effects of Gl-Ps in breast cancer [221].In 2015, Habijanic et al. reported on the effects of Gl-Ps in modulating cytokine responses and lymphocyte activity [222].Several studies have delved into the mechanisms of action of G. lucidum compounds in cancer treatment, such as those published by Ahmad F., Sohretoglu et [13,[223][224][225].The triterpene compounds (Gl-Ts) identified in G. lucidum exhibit significant anticancer effects.Both Min et al. in 2000 and Gao et al. in 2002 reported that triterpene compounds displayed cytotoxic effects against tumor cells [226,227].In 2004, Lin et al. investigated the actions of Gl-Ps from aqueous extracts and Gl-Ts from alcoholic extracts of G. lucidum, evaluating their angiogenic effects [228].Li et al. conducted research on ganoderic acid X, which acts by inhibiting topoisomerases and inducing apoptosis [229].In Table 9, we have compiled the effects of essential compounds (Gl-Ps and Gl-Ts) in G. lucidum that significantly contribute to its anticarcinogenic activity.Antitumor action of a novel polysaccharide with an estimated average molecular weight of 1.5 × 104 Da In vivo antitumor activity tests showed that Gl-Ps could significantly inhibit S180 tumor growth in mice.No drug-related toxic reactions were observed.[225] 16 Gl-Ts Ganoderic acids from spores and their cytotoxicity The cytotoxicity of the compounds isolated from the Ganoderma spores was carried out in vitro against Meth-A and LLC tumor cell lines.[227] 17 Gl-Ts Anticancer study of lucialdehydes B, C (2,3), ganodermanonol, and ganodermanondiol Cytotoxic mechanism.Lucialdehyde C exhibited the most potent cytotoxicity against CLL, T-47D, sarcoma 180, and Meth-A tumor cells.[227] 18 Gl-Ps Gl-Ts Antitumor effect of aqueous extract; cytotoxic activity of alcoholic extract Manifestation of a significant antitumor effect in several tumor-bearing animals; manifestation of an anti-angiogenic effect that may be involved in the antitumor activity. [228] 19 Gl-Ts Ability of ganoderic acid X (GAX) to inhibit topoisomerases and interfere with apoptosis Mechanisms of chromosomal DNA degradation, cancer cell apoptosis, mitochondrial membrane disruption, and caspase-3 activation have been elucidated upon GAX treatment of HuH-7 human hepatoma cells. [229] 20 Gl-Ts Cytotoxicity of GA-T on different human carcinoma It was shown in vivo to significantly inhibit proliferation of lung cancer cells by inducing apoptosis by GA-T [230] 21 Gl-Ts The effect of ganoderic acids A, F, and H on breast cancer cells was evaluated GA-A, GA-F, and GA-H suppressed cell proliferation, colony formation, and invasive behavior of MDA-MB-231 cells.They have biological effects by inhibiting transcription factors AP-1 and NF-κB. [231] 22 GA-T Studies of anti-invasive and antimetastatic mechanisms of GA-T in vitro in lung cancer GA-T dose-dependently inhibited 95-D cell migration by wound healing assay, promoting cell aggregation and inhibiting cell adhesion to the extracellular matrix (ECM).GA-T prevents tumor metastasis in highly metastatic lung carcinoma. [232] 23 Gl-Ts.Anticancer, anti-inflammatory, and antimetastatic activities of G. lucidum extracts Gl-Ts from G. lucidum reduces the production of IL-8, IL-6, MMP-2, and MMP-9 in breast cancer and melanoma cells.They decrease cancer cell viability in a time and dose-dependent manner. [233]

GL-Ts
Investigation of Gl-Ts with activity in inhibiting growth of pulmonary carcinoma metastates and suppressing colonic inflammation The triterpene extracts exhibit inhibitory activity against foodborne carcinogen-induced mouse colon carcinogenesis.All suppressive functions were enhanced by high doses of triterpene extract.The cytotoxic effects of Gl-Ts on human colon cancer cells SW480, SW620, SW116, and mouse embryonic fibroblast cells NIH3T3 were studied.Compounds isolated from NTF acted as antitumorals by inducing apoptosis.[235] 27 GlSO Mechanistic investigation of the anticancer-gene effect of GlSO (G.lucidum spore oil) on mammary cancer cells Growth of MDA-MB-231 cells, in vitro, were inhibited by treatment with GlSO (0.2, 0.4, and 0.6 µL/mL).In vitro, GlSO increased Bax and caspase-3 expression but did not affect caspase-8 expression.[236] 27 Gl-Ts Anticancer potential of G. lucidum against prostate cancer (PC-3) G. lucidum has been shown to prevent prostate cancer cell growth and stimulate apoptosis in PC-3 cells by preventing STAT-3 translocation (signal transduction and activation of transcription). [237] 28 Gl-Ts Effects on colorectal cancer.Involves suppression of NF-κB-regulated inflammation and carcinogenesis In vitro administration of GLSF extract at non-toxic concentrations to mice inoculated with CT27 tumor cells significantly potentiated paclitaxel-induced growth inhibition and apoptosis in CT27 and HCT-15 cells. [238] 29 Gl-Ps, Gl-Ts Evaluation of the effects on skin carcinogenesis analyzed on JB6 cells in SKH-1 mice Reduced incidence and multiplicity of skin tumors.In tumor-free skin tissue of mice, Gl-Ps and Gl-Ts attenuated UV-induced epidermal thickening.Gl-SF increased CD8 and Granzyme B expression.Tang et al. (2006) reported the effects of ganoderic acid T in inhibiting lung cancer tumors (95-D), inducing apoptosis, and arresting the cell cycle at the G(1) phase [230].Gl-Ts-type triterpenes and ganoderic acids A, F, and G were investigated in 2008 by Jiang et al. for their effects on breast cancer [231].In 2009, Trajkovic et al. documented the action of triterpenes in B-16 melanoma, while Xu et al. studied ganoderic acid T and its effects on lung cancer [34,232].In 2017, Barbieri et al. published data on the inhibition of IL-8, IL-6, MMP-2, and MMP-9 release in cancer cells under pro-inflammatory conditions in breast cancer and melanoma cells, and Ye et al. disclosed data on the effects of ganoderic and lucidenic acids in inhibiting the growth of lung carcinoma metastases and suppressing colon inflammation [233,234].In 2020, the anticancer effects of triterpene fractions extracted from G. lucidum on SW620 human colorectal cancer cells were reported in vitro by Li et al. [235].The action of Gl-Ts against various cancers has been studied by Jiao et

Possible Mechanisms in Anticancer Actions
The medical world has shown significant interest in establishing possible mechanisms of anticancer activity, especially as the literature provides ample evidence regarding the actions of G. lucidum biocomponents against various forms of cancer.In 2018, Ahmad F. systematized the possible mechanisms by which G. lucidum bio-compounds participate in cancer treatment [13].The steps considered by Ahmad include the activation of the host cell immune response, induction of cell differentiation, inhibition of angiogenesis, direct cytotoxicity to tumor cells, inhibition of urokinase-type plasminogen activator and receptor expression in cancer cells, and inhibition of phase II metabolizing enzymes [13].
Kao et al. elucidated the mechanisms of the anticancer action of G. lucidum compounds by discussing the distinct effects of polysaccharide and triterpene compounds separately, as well as their combined effects (see Figure 9) [35].
Antioxidants 2023, 12, x FOR PEER REVIEW 20 of 36 Tang et al. (2006) reported the effects of ganoderic acid T in inhibiting lung cancer tumors (95-D), inducing apoptosis, and arresting the cell cycle at the G(1) phase [230].Gl-Ts-type triterpenes and ganoderic acids A, F, and G were investigated in 2008 by Jiang et al. for their effects on breast cancer [231].In 2009, Trajkovic et al. documented the action of triterpenes in B-16 melanoma, while Xu et al. studied ganoderic acid T and its effects on lung cancer [34,232].In 2017, Barbieri et al. published data on the inhibition of IL-8, IL-6, MMP-2, and MMP-9 release in cancer cells under pro-inflammatory conditions in breast cancer and melanoma cells, and Ye et al. disclosed data on the effects of ganoderic and lucidenic acids in inhibiting the growth of lung carcinoma metastases and suppressing colon inflammation [233,234].In 2020, the anticancer effects of triterpene fractions extracted from G. lucidum on SW620 human colorectal cancer cells were reported in vitro by Li et al. [235].The action of Gl-Ts against various cancers has been studied by Jiao et

Possible Mechanisms in Anticancer Actions
The medical world has shown significant interest in establishing possible mechanisms of anticancer activity, especially as the literature provides ample evidence regarding the actions of G. lucidum biocomponents against various forms of cancer.In 2018, Ahmad F. systematized the possible mechanisms by which G. lucidum bio-compounds participate in cancer treatment [13].The steps considered by Ahmad include the activation of the host cell immune response, induction of cell differentiation, inhibition of angiogenesis, direct cytotoxicity to tumor cells, inhibition of urokinase-type plasminogen activator and receptor expression in cancer cells, and inhibition of phase II metabolizing enzymes [13].
Kao et al. elucidated the mechanisms of the anticancer action of G. lucidum compounds by discussing the distinct effects of polysaccharide and triterpene compounds separately, as well as their combined effects (see Figure 9) [35].They showed that polysaccharide compounds (Gl-Ps) act through three mechanisms [35]: by enhancing the host immune response, by stimulating macrophage activity, and by stimulating T-lymphocytes and natural killer (NK) cells.Triterpenes (Gl-Ts) act through five mechanisms: -G1-phase cell cycle arrest by inhibition of β-catenin; -Inhibition of protein kinase C (PCK), which generates G2-phase cell cycle inhibition; -Induction of apoptosis in cancer cells via the mitochondrial pathway, followed by activation of caspase cascades; -Preventing tumor metastasis by inhibiting MMP-9 and interleukin IL-8 and by degrading the extracellular matrix (ECM); -Suppressing the secretion of anti-inflammatory cytokines [240].
Together, the two major classes of bio-compounds, Gl-Ps and Gl-Ts, share two common mechanisms, namely: I.
Antioxidant actions by reducing oxidative stress generated by free radicals and reactive oxygen species (ROS) through the actions of antioxidant enzymes [35].II.Suppressing angiogenesis and inhibiting nitric oxide production.

Other Biological Actions Involved in Anticancer Activities
The antitumor activity of G. lucidum bio-compounds is based on the differential effects of other biological activities generated by G. lucidum bio-compounds, as reported by Cör et al. [122,153]

Effects of Gl-Ps on T-and B-Lymphocytes
Numerous studies have been reported that highlight the activating role of Gl-Ps (F3 fraction) on T-lymphocytes by increasing interleukin production: IL-1, IL-2, IL-6, and IL-12, and increasing the expression of IFN-γ and INF-α.Additionally, they enhanced DNA synthesis in mouse spleen cells, as reported by Chen et al. [213] and Wang et al. [24].Gl-Ps from G. lucidum can activate PKC and PKA protein kinases in murine T-lymphocytes, according to Sohretoglu et al. [223].The effect of Gl-Ps on B-lymphocytes is also crucial for tumor immunity.For example, Gl-Ps can activate B-lymphocytes by enhancing their proliferation and differentiation, leading to the production of substantial amounts of immunoglobulins in mice.Furthermore, Gl-Ps can directly stimulate the expression of PKCα and PKCγ in B-lymphocytes, as reported by Zhang et al. [242].Activated B-lymphocytes increase the production of immunomodulatory substances, such as IL-1β and TNF-α, and reactive nitrogen species, such as NO [242].
Effect of Gl-Ps on Dendritic Cells Dendritic cells (DCs) are professional antigen-presenting cells involved in the initiation of the primary T-lymphocyte immune response [223].Stimulation of dendritic cell maturation by Gl-Ps from G. lucidum was documented by Sanodiya et al.

Effect of Gl-Ps on Macrophages
Sohretoglu et al. demonstrated that Gl-Ps activate macrophages in vitro and elevate the levels of various cytokines, including IL-1β, tumor necrosis factor (TNF-α), IFN-γ, and IL-6 in the culture medium.This activation significantly increases macrophage phagocytosis and enhances macrophage-mediated tumor cytotoxicity [223].Zhang et al. showed that the in vivo treatment of S180 sarcoma-bearing mice with Gl-Ps activated bone marrow-derived macrophages, inducing the production of immunomodulatory compounds such as IL-1β, TNF-α, and nitric oxide (NO) [242].Hsu et al. elucidated the role of caspases in macrophage F3 fraction-induced Gl-Ps from G. lucidum spores [246].Guo et al. discovered a novel watersoluble polysaccharide within G. lucidum spores that acts as an inducer of TNF-α and IL-6 secretion in murine peritoneal macrophages [247].The in vivo administration of extracts from G. lucidum spores potentiated the proliferative response of splenocytes and induced antitumor activity against lung cancer in mice [247].Hsu et al. reported that Gl-Ps induces increased secretion of the inflammatory cytokine IL-1 and stimulates the expression of pro-IL-1 and IL-1-converting enzymes in human and murine macrophages, an association linked with its anticancer activity [248].
Effect of Gl-Ps on Natural Killer (NK) Cells Altfeld et al. defined the role of (NK) natural killer cells in innate immunity [249].Chien et al. demonstrated that treatment with Gl-Ps resulted in increased monocyte, macrophage, and NK cell populations in human umbilical cord blood [250].Wang et al. studied the effects of the bio-compounds from G. lucidum that, upon oral administration to mice, improved NK cell and phagocytosis activities and increased cytokine levels [251].In additional research by Zhu et al., it was shown that Gl-Ps accelerated the recovery of bone marrow cells, red blood cells, and white blood cells, as well as splenic NK and NKT killer cells, and enhanced T-and B-lymphocyte proliferative responses [215].The application of Gl-Ps treatments is recommended in cancer chemotherapy only at low doses [215].

Anti-Proliferative, Cytotoxic, and Apoptosis-Increasing Activities
Ganoderic bio-compounds have demonstrated various anti-proliferative and cytotoxic effects in studies regarding the treatment of different types of cancer [252][253][254][255].The anti-proliferative effects of Gl-Ts compounds in G. lucidum manifest through cell cycle arrest [252,253].Gl-Ts compounds can arrest the cell cycle in the G1 phase by inhibiting the β-catenin pathway, as reported by Wu et al., and in the G2/M phase by suppressing protein kinase C (PKC) activity, as reported by Lin et al. [252,253].Jedinak et al. reported that ganodermanontriol inhibited the proliferation of HCT116 and HT-29 colon cancer cells by inhibiting β-catenin [254].Li et al. (2005) identified ganoderic acid X as a compound that can arrest the cell cycle by inhibiting topoisomerase [229].Chen et al., in 2010, reported that ganoderic acid T (GA-T) exhibits anti-proliferative effects against cancer cells in vitro and against metastasis in vivo [255].In 2008, Chen et al. reported in the wound vacuolization assay that ganoderic acid Me (GA-Me), administered in a dose-and time-dependent manner, inhibited tumor invasion and cell adhesion to the extracellular matrix (ECM) [256].GA-Me suppressed master metalloproteinases at the mRNA and protein levels in 95-D cells and is considered a potent antimetastatic carcinoma inhibitor [256].Hsu et al. studied the anti-proliferative effects of lucidenic acids in human leukemic HL-60 cells [257].
Tang et al. conducted a study on the anti-proliferative effect of ganoderic acid T against cancer cells and observed enhanced cytotoxicity in lung cancer [230].Triterpene compounds can induce apoptosis of cancer cells via the mitochondria-dependent pathway, followed by caspase activation, as reported by Kao et al. andLiu et al. in 2011 and2012 [35,258,259].Zhou et al. investigated the cytotoxic effects of GA-Me in human colon carcinoma, observing a dose-dependent pattern, and determined that the anticancer bioactivity of GA-Me was mediated through induced apoptosis [260].

Anti-Inflammatory Activities
Several inflammatory mediators, such as TNF-α, IL-6, TGF-β, and IL-10, have been shown to play roles in cancer initiation and progression [261,262].G. lucidum biocomplexes have demonstrated dose-dependent anti-inflammatory effects [223].Administration of the triterpene extract suppressed inflammatory cytokine secretion in macrophages with a significant reduction in inflammation in the affected tissue, as reported by Dudhgaonkar et al. in 2009 [263].Joseph et al. found that Gl-Ps administration resulted in a 58% inhibition of inflammation, as assessed by carrageenan-induced (acute) and formalin-induced (chronic) inflammation assays [37].

Anti-Angiogenic Activities
Angiogenesis, the process by which new vasculature is formed from pre-existing vasculature, plays a key role in tumor growth and metastasis as well [264].Nitric oxide is known to be an angiogenesis-inducing agent in tumors, promoting capillary formation within the tumor and allowing it to expand.In 2004, Cao et al. reported the existence of a peptide in G. lucidum that significantly reduced microvessel formation, as detected by the chorioallantois membrane assay [265].Stanley et al. (2005) found that the G. lucidum extract prevented capillary morphogenesis by inhibiting the secretion of angiogenic factors VEGF and (TGF)-β1 [38].Cao et al. (2006) demonstrated that the G. lucidum extract contains a polysaccharide peptide that exhibits anti-angiogenic activity by inhibiting nitric oxide production, subsequently suppressing cell multiplication in a dose-dependent manner [95].

Antioxidant Activities
Excess free radicals adversely affect bases in the nucleic acid structure, amino acids in the protein structure, and double bonds in unsaturated fatty acids, leading to oxidative stress, which is responsible for the alteration of DNA, RNA, proteins, and lipids.Hsieh et al. reported that bio-compounds from G. lucidum can exert chemopreventive effects through their antioxidant properties, such as free radical scavenging, as well as the ability to affect phase II detoxification enzymes [39].Other authors, such as Smina et al., studied the antioxidant activity of Gl-Ts and demonstrated that they can reduce free radicals in cancer cells.[266].In another study, Smina et al. reported the effect of total triterpenes from G. lucidum on the intracellular levels of reactive oxygen species (ROS) and the activities of endogenous antioxidant enzymes in spleen lymphocytes, highlighting their role in reducing radiation-induced oxidative DNA damage in spleen cells [267].In 2001 and 2003, Lu et al. highlighted the beneficial contribution of the polysaccharides extracted from G. lucidum mycelium in reducing ROS-induced oxidative damage [268,269].Lee et al. reported the existence of an amino polysaccharide compound in G. lucidum that can inactivate hydroxyl and superoxide anion radicals [270].Other authors, such as XiaoPing et al. and Zhao et al., studied Gl-Ps and showed that these bio-compounds could be beneficial for glutathione peroxidase and reduce malonaldehyde levels in rats with cervical carcinoma and mice exposed to γ-irradiation [49,271].

Toxicity and Safety
Although there are numerous published studies on the beneficial effects of G. lucidum, there is also relatively little information reporting the toxic effects in humans.In this regard, Ahmad F. highlighted human sensitisation to Ganoderma antigen, reported in the USA, leading to allergic reactions [13,155].When undergoing G. lucidum treatment, special attention should be paid to potential interactions with other drugs.Diabetic patients or those being treated with anticoagulants or antiplatelet drugs require special caution when being administered G. lucidum, as the anticipated effects may be altered [272].
In cancer therapy, although G. lucidum has been utilized as an anticancer agent, caution is still required when using it in conjunction with chemotherapy due to potential toxicity.Plasma concentrations of G. lucidum should be carefully monitored to detect elevated, toxic levels [273].In an in vitro study, G. lucidum extracts were found to have toxic effects when exposed to cells at concentrations higher than those required for stimulatory results, resulting in a significant reduction in cell viability in a number of cell lines [274].G. lucidum exhibits antihypertensive activity and may potentiate the effects of antihypertensive drugs [275].Gl-Ps from G. lucidum have antibacterial activity and can enhance the activity of some antibiotics (e.g., tetracycline and cefazolin) [276].

Conclusions
G. lucidum bio-compounds are regarded as valuable in alternative cancer treatments based on non-natural products.The present work has compiled available data from various in vitro and in vivo studies on G. lucidum bio-compounds and their beneficial effects in anticancer treatment through their biological actions, such as anti-proliferative, antioxidant, immunomodulatory, anti-inflammatory, and anti-angiogenic effects.This paper presents information on the active bio-compounds in G. lucidum to obtain conclusive data and confirm their benefits regarding the mechanisms of anticancer action.Additionally, understanding the mechanisms of anticancer action, combined with other biological anticancer actions exerted by both the main bioactive compounds Gl-Ps and Gl-Ts and other compounds described in the paper (proteins, vitamins, metals, sterols, fatty acids, and nucleotide compounds), is necessary for targeted use in anticancer treatments.It is also crucial to comprehend that the biocomponents of G. lucidum are directly influenced by several factors, including the origin and culture medium, environmental conditions, temperature, humidity, and the quality of the environment from which the fungus originates.Therefore, further experimental, epidemiological, and clinical studies are needed to characterize the interactions of the administration of G. lucidum forms with different conventional anticancer drugs.More research is needed to combine G. lucidum bio-compound treatments with chemotherapy.Extensive pharmacological studies are also necessary to establish optimal dosages and assess the efficacy and safety of administration.Moreover, it is important to extend the research to identify metabolite subtypes that support the observed bioactivities, aiming to establish anticancer therapy procedures that promote general health and longevity.

Figure 2 .
Figure 2. Different cultivation methods for fruit body and mycelium of Ganoderma lucidum, adapted from [14].

Figure 2 .
Figure 2. Different cultivation methods for fruit body and mycelium of Ganoderma lucidum, adapted from [14].

Figure 2 .
Figure 2. Different cultivation methods for fruit body and mycelium of Ganoderma lucidum, adapted from [14].

Figure 3 .
Figure 3. Bioactive compounds in G. lucidum and biological actions involved in anticancer activity, adapted from Ahmad [13].

3. 2 .
Polysaccharide Content of G. lucidum Numerous studies have identified the existence of several types of polysaccharides in the fruiting body, spores, or mycelium of G. lucidum, as reported by Liu et al. and Lin et al. [78,79].Polysaccharides represent a class of macromolecules with diverse structures and a wide range of physicochemical properties and biological actions, according to studies by Ferreira et al., Yu et al., and Giavasis [80-82].According to Ahmad, Bhat et al., and Liu et al., these polysaccharide compounds are considered some of the most potent bioactive metabolites with antitumoral effects due to their biochemical structure [13,18,78].Polysaccharide compounds (Gl-Ps) have a rich history and have been extensively studied in recent years by Sanodiya et al., Parepalli et al., and Chen et al. [27,62,83].

Figure 3 .
Figure 3. Bioactive compounds in G. lucidum and biological actions involved in anticancer activity, adapted from Ahmad [13].

3. 2 .
Polysaccharide Content of G. lucidum Numerous studies have identified the existence of several types of polysaccharides in the fruiting body, spores, or mycelium of G. lucidum, as reported by Liu et al. and Lin et al. [78,79].Polysaccharides represent a class of macromolecules with diverse structures and a wide range of physicochemical properties and biological actions, according to studies by Ferreira et al., Yu et al., and Giavasis [80-82].According to Ahmad, Bhat et al., and Liu et al., these polysaccharide compounds are considered some of the most potent bioactive metabolites with antitumoral effects due to their biochemical structure [13,18,78].Polysaccharide compounds (Gl-Ps) have a rich history and have been extensively studied in recent years by Sanodiya et al., Parepalli et al., and Chen et al. [27,62,83].

Figure 7 .
Figure 7. Structure of ganoderic acids from G. lucidum, adapted from [122].Murata et al. (2019) identified and extracted a novel compound with a lanostane triterpene structure from G. lucidum using NMR and MS physicochemical analysis [139].Studies presented by Yue et al. in 2008 and 2010, as well as those by Zhang et al. and Cheng et al., revealed the cytotoxic effects exerted by ganoderic acids from G. lucidum, which alter proteins involved in cell proliferation and cell death in carcinogenesis or oxi-

3. 5 .
Protein, Lectin, and Amino Acid Content 3.5.1.Proteins and Peptides in G. lucidum Proteins constitute a distinct category of biochemically active molecules in G. lucidum, as demonstrated by Cör et al. in 2018 [153].Numerous studies have reported various biological effects of the primary fungal protein, Ling-zhi-8 (LZ-8).Structural reports were published by Huang et al. in 2009, and the immunomodulatory actions of LZ-8 were documented by Hsu et al. in 2013, Lin et al. in 2014, and Yang et al.

Figure 9 .
Figure 9. Mechanisms of action of polysaccharide and triterpene extracts isolated from G. lucidum with anticancer effect, adapted from [35].Figure 9. Mechanisms of action of polysaccharide and triterpene extracts isolated from G. lucidum with anticancer effect, adapted from [35].

Figure 9 .
Figure 9. Mechanisms of action of polysaccharide and triterpene extracts isolated from G. lucidum with anticancer effect, adapted from [35].Figure 9. Mechanisms of action of polysaccharide and triterpene extracts isolated from G. lucidum with anticancer effect, adapted from [35].
[27].Lai et al. reported that treatment with Gl-Ps F3 fraction extract improved the mixed lymphocyte response and stimulated the production of ten cytokines and six chemokines [243].Jan et al. outlined the immunomodulatory activity of Gl-Ps, including the activation and maturation of DCs, as evidenced by increased cytokine production (IL-12, IL-6, IL-23, and IL-10) [244].Chan et al. reported that treatment of monocytic leukemic cell lines with Gl-Ps resulted in increased leukocyte HLA-DR antigen expression and induced leukemic cell differentiation through increased superoxide production and cell cycle arrest [245].

Table 1 .
Proximate composition of the fruiting body of G. lucidum from different country origins, reported as a percentage.
Wang et al. (2011)researchers who have used diluted saline solution or alkaline acid solutions for polysaccharide extraction, as reported byWang et al. (2011).Other extraction methods that were applied included microwaves, ultrasound, or enzymatic methods, as in the studies ofLinet al. (2005), Huang et al. (2010), Zhao et al. (2010), and Leong et al. (2021) [86-90].After filtering, the solution is subjected to precipitation with alcohol or acetone to obtain crude polysaccharides [84].Polysaccharide purification can be achieved by several techniques, such as ethanol fractionation or different column chromatographic techniques, as illustrated by Chen et al. (2008), Huang et al. (2011), and Jiang et al. (2012)

Table 2 .
Structural characteristics, extraction, and fractionation techniques of polysaccharides from G. lucidum.

Table 3 .
Minerals of G. lucidum fruiting body.Sterols with the chemical formula C 17 H 28 O are a group of steroids that are cyclic secondary monohydric alcohols.Since 2002, Ma et al. have reported three new lanostanoids and two ergostane sterols, which were isolated by spectroscopic methods [181] et al. (2022)ndher insights were provided byYu et al. in 2021 and 2023, where they specifically documented the effects of proteoglycans derived from G. lucidum[176][177][178].Huang et al. (2022)reported a novel pentapeptide in the mycelium of Ganoderma spp. that demonstrates antioxidant properties[179].3.5.2.Lectins from G. lucidumLectins are glycoproteins found in the fruiting body of G. lucidum.Lectins are carbohydrate-binding proteins that have been classified according to their origin and structure.In 2007, Thakur et al. isolated and purified a group of lectin-structured proteins with a weight of 114 kDa from the fruiting bodies of G. lucidum[180].In 2011, Girjal et al. isolated a new bioactive lectin from G. lucidum, which also exhibited hemagglutinating activity against both human and animal erythrocytes[181].Nikitinaet al. (2017) investigated lectins in mycelia and detected hemagglutinating activity in culture medium and in a crude mycelial extract of G. lucidum [182].Recent research conducted by Yousra et al. has demonstrated varying binding patterns of lectins from G. lucidum with glycan moieties.These patterns play a crucial role in influencing biological activities, including antitumor, antiviral, and immunomodulatory effects [183].3.5.3.Amino Acid Content of G. lucidum The amino acid content has been documented by several authors.According to the research of Sanodiya et al., Yousra et al., and Deepalakshmi et al., the most abundant amino acid was glutamic acid [27,183,184].This was followed by aspartic acid, glycine, and alanine.The amino acids in G. lucidum have also been analyzed by Zhang et al. in 2018, who reported 18 different types of amino acids [185].It should be noted that factors such as the origin of the tested samples and the species of fungus, as well as different analysis techniques, may result in different amino acid values.Compounds with antioxidant properties from G. lucidum have been analyzed in numerous studies.Thus, Kim et al. (2008), Sheikh et al. (2014), Lin et al. (2015), and Veljović et al. (2017) presented data on the chemical compounds of G. lucidum [186-189].The amino acid values for G. lucidum, as reported by Sanodiya et al. in 2009 and Deepalakshmi et al. in 2011, are presented in Table 4 [27,184].

Table 4 .
Amino acids content in Ganoderma lucidum mushroom.

Table 5 .
Compounds with antioxidant activity from G. lucidum.Ostek et al. presented more data on several phenolic acids present in G. lucidum responsible for antioxidant activity, which are presented inTable 6 [192].

Table 7 .
Antioxidant capacity in the extract of G. lucidum.

Table 9 .
Anticancer activity of polysaccharides and triterpenes from G. lucidum.
. Other researchers, such as Sharma et al., Ahmad, and You et al., have also conducted studies on the various factors that can lead to cancer [137,155,216].Additionally, researchers like Xu et al., Fu et al., and Hapuarachchi et al. have analyzed various biological actions associated with cancer [217,225,241].4.3.1.Immunomodulatory Activities Anticancer activity based on immunomodulation activity has been reported by several researchers, such as Bao et al., Ooi et al., Moradali et al., and Lin et al. [94,106,109,168].